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Pyrosequencing
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DRdoubleB
Pyrosequencing animation
Nucleotides are added, one at a time. If incorporated, the pyrophosphate release is converted to a flash of light. The dispensation order is shown at the bottom; if there's a peak, the nucleotide was incorporated. If there are multiple of the same nucleotide in a row, they'll all be incorporated at once, giving a higher peak. Nucleotides must ...
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